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Fig. 5
Whole-mount immunostaining of T4 and TG. Uninjected (Uninj) (A), injected with standard control-MO (scMO) (C and E) and wwtr1-MO (B, D, F) 4 dpf larvae were immunolabeled for T4 (A-D) and TG (E and F). Thyroid follicles are reduced in number (A-D) and not restricted to the ventral midline in Taz-deprived larvae (C-F). Arrowheads indicate thyroid follicles. Scale bar represents 200 µm (A, B, E, F) and 100 µm (C and D). Anterior is to the left in ventral (A-D) and lateral views (E and F).
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Reprinted from Mechanisms of Development, 138 Pt 3, Pappalardo, A., Porreca, I., Caputi, L., De Felice, E., Schulte-Merker, S., Zannini, M., Sordino, P., Thyroid development in zebrafish lacking Taz, 268-78, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.