|
Fig. S1
huC regulatory sequences drive expression in some OPCs. Tg:olig2:GFP stably transgenic embryos were injected with a construct encoding huC:mCherry-CAAX, which labeled cells expressing huC regulatory sequences with membrane-tethered mCherry (B, red). (A-C) Representative embryo showing (A) GFP in oligodendrocytes and motor neurons, (B) membrane tethered mCherry, and (C) merged image. OPCs and motor neurons were distinguished by morphology and position. In 3 of 38 injected embryos, some OPCs co-expressed GFP and membrane-tethered mCherry (arrow), indicating that the HuC construct may be expressed in a subset of cells of the oligodendrocyte lineage. (A′-C′) These images show magnified views of boxed regions in A-C.