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Fig. S3 Secondary antibody validation in zebrafish embryo samples at the different developmental stages. GPx4 antibody was omitted and unspecific secondary antibody binding to embryonic tissues was tested. We found that when embryos are processed with the same protocol but the primary antibody is omitted the secondary antibody give almost null signal when visualized with the same confocal imaging settings and during the same imaging sessions. Scale bar 250 µm.
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Reprinted from Gene expression patterns : GEP, 19(1-2), Mendieta-Serrano, M.A., Schnabel-Peraza, D., Lomelí, H., Salas-Vidal, E., Spatial and temporal expression of zebrafish glutathione peroxidase 4 a and b genes during early embryo development, 98-107, Copyright (2015) with permission from Elsevier. Full text @ Gene Expr. Patterns