|
Fig. 2 Immunostaining and histochemistry for Hapln1a-ECM components during the time course of regeneration. Longitudinal fin sections were treated with the respective primary antibodies and detected using the corresponding secondary antibody conjugated with Alexa Fluor– 488 (green). Propidium iodide (nuclei) is used as the counter stain (red). For each time point the percentage of sections showing similar expression pattern is denoted in each panel (n = 40–65 sections). (A) Immunostaining for Hapln1a; (B) histochemical detection of HA using biotin-HA binding protein; (C) immunostaining for Acan, the arrowhead identifies the joints and (D) immunostaining for Vcan. (E) The graph illustrates the overall change in the expression level during the time course of regeneration for each component. Efforts to compare expression levels between components were not completed. Arrows identify the basal layer of epithelium (BLE); yellow arrowhead identifies lepidotrichia and yellow arrow identifies actinotrichia; m, mesenchyme; e, epidermis; dpa, days post amputation. Scale bar is 20 µm.
Reprinted from Gene expression patterns : GEP, 19(1-2), Govindan, J., Iovine, M.K., Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration, 21-9, Copyright (2015) with permission from Elsevier. Full text @ Gene Expr. Patterns