ZFIN Image: Won et al., 2015, Fig. 2

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Figure Caption

Fig. 2

lnx2a knockdown causes defects in the exocrine pancreas. (A) Schematic drawing of the lnx2a locus containing 11 exons, and the Lnx2a protein (737 aa) containing a RING-finger domain (RF), the Numb binding NPAF motif (F), and four PDZ domains. The translation initiation site is located in exon 2, which encodes the RING-finger domain. The lnx2a-MO targets the splice donor site of exon 2. Primers in exon 1 (F1) and exon 11 (R1) are shown, as is the epitope for the Lnx2a antibody in the third PDZ domain. (B and C) The lnx2a-MO (5 ng) leads to inhibition of exocrine markers (try, ptf1a), but not endocrine markers (ins, glu), as seen by two-color WISH at 60 hpf. (D and E) Quantification of marker expression; effects were classified as strong, partial, and unaffected. MO, lnx2a-MO; UN, uninjected. (Scale bar: 100 µm.)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA