ZFIN Image: Sun et al., 2015, Fig. S7

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Figure Caption

Fig. S7

Knockdown of Bnip-h Impairs Cholinergic Signaling and Motility in Zebrafish, Related to Figure 7

(A) The structures of slow and fast muscles are disrupted by Bnip-h knockdown. The slow muscle fibers were stained with anti-myosin heavy chain 1/2/4/6 antibody (F59). The fast muscle fibers were stained with anti-myosin light chain 1/3f antibody (F310). Scale bar, 50 µm.

(B, C and D) The escape response to tapping is blocked by Bnip-h, Acl or Chat knockdown. The larvae at 72hpf were placed in 10cm petri dishes, and the tapping stimulus was applied to one side of the dish with around 5 s intervals. The movement of larvae was recorded over 30 s. Percentage (%) of swimming larvae is presented. n = 30- 50 fishes/category from three experiments. Error bars represent SD. **p < 0.01.

(E) The spontaneous movement of bnip-h morphants at 72 hpf is reduced. Similar amounts of larvae were placed into eppendorf tubes. The number of swimming larvae across the half depth of the water is quantified over 30 s. See also Movies S4-S5.

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Acknowledgments

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Reprinted from Developmental Cell, 34(5), Sun, J., Pan, C.Q., Chew, T.W., Liang, F., Burmeister, M., Low, B.C., BNIP-H Recruits the Cholinergic Machinery to Neurite Terminals to Promote Acetylcholine Signaling and Neuritogenesis, 555-68, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell