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Fig. S1 The bacterial artificial chromosome (BAC) reporter line Tg(nkx6.1:eGFP) mirrors the expression of the endogenous nkx6.1 gene. A Schematic representation of the 55 to +95 kb nkx6.1:eGFP BAC transgene. This BAC includes sequence blocks that are highly conserved amongst vertebrates (black boxes) located from 11 to 77 kb downstream of the nkx6.1 gene. By BAC recombineering using galK selection, the eGFP cassette (green box) was introduced into exon 1, replacing the beginning of the nkx6.1 open reading frame (aa 1 to 149). B Epifluorescence microscopy images of the immunodetection of endogenous Nkx6.1 (red) and GFP (green) in Tg(nkx6.1:eGFP) embryos. Lateral views of 48 hpf embryos with the anterior part to the left. C Confocal projection images of whole mount fluorescent in situ hybridization on a Tg(nkx6.1:eGFP) embryo showing that the gfp transcripts are not expressed in the insulin+ cells at 20 hpf. D Confocal projection images of whole mount fluorescent in situ hybridization on a Tg(nkx6.1:eGFP) embryo showing that the gfp transcripts are not expressed in the isl1+ cells at 30 hpf. Scale bar = 15 µm. P pancreas.