ZFIN Image: Campbell et al., 2015, Fig. S3

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Figure Caption

Fig. S3

Injection of control RNA has no effect on number of PGCs in WT or Mkif5Ba mutant embryos.

(A-B) The is no difference in the number of nanos3-positive PGCs at 4hpf in embryos injected with either 800pg of a control RNA encoding Cherry protein (A; 3.46±0.26 PGCs in n=37 embryos) or 800pg of ha-kif5Ba RNA (B; 3.56±0.25 PGCs in n=32 embryos)

(C-D) While injection of 800pg of a control RNA encoding Cherry protein has no effect on the number of PGCs in Mkif5Ba mutants (C; zero PGCs in n=42/43 embryos, one PGC in n=1/43 embryos) injection of 800pg of ha-kif5Ba leads to the appearance of one PGC in a subset of Mkif5Ba mutants (D; one PGC visible in 13/85 embryos). Compare with uninjected Mkif5Ba mutants (zero PGCs in n=72/73 embryos, one PGC in n=1/73 embryos).

Acknowledgments

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