Fig. 6

Fig. 6

Maternal kif5Ba localizes dorsal determination components in activated eggs. (A-D2) sybu is vegetally localized at 2mpa (A,B) and at 45mpa (C,D) in both WT (A,C) and Mkif5Ba mutant (B,D) activated eggs. However, sybu is more dispersed at 2mpa (B) and appears less abundant in most Mkif5Ba eggs at 45mpa (D,D′). [2mpa: n=30/30 for Mkif5Ba^ae11/ae12 (N=2 females), n=29/29 for Mkif5Ba^ae12/ae12 (N=1 female), n=63/63 for WT (N=3 females) have vegetally localized sybu; 45mpa: quantification in panels.] (E) qRT-PCR reveals significantly more abundant sybu RNA in Mkif5Ba mutants at 2mpa, although this RNA excess is not maintained at 45mpa. Error bars show mean±s.d.; Student′s t-test, *P=0.0219. (F-I) grip2a is vegetally localized at 2mpa (F,G) and shifts asymmetrically by 45mpa (H,I) in both WT (F,H) and Mkif5Ba mutant (G,I) activated eggs. [2mpa: n=37/37 for Mkif5Ba^ae11/ae12 (N=2 females), n=23/23 for WT (N=2 females) have vegetally localized grip2a; 45mpa: see N for quantification.] (J-M) wnt8a is vegetally localized at 2mpa in both WT (J) and Mkif5Ba mutant (K) activated eggs. However, wnt8a is asymmetric at 45mpa in WT (L) but not Mkif5Ba mutant (M) activated eggs. [2mpa: n=24/24 for Mkif5Ba^ae11/ae12 (N=2 females), n=29/29 for WT (N=2 females) have vegetally localized wnt8a; 45mpa: see N for quantification.] (N) Fraction of eggs exhibiting dorsally translocated RNA at 45mpa. Number at base of columns indicates the number of eggs assayed. Chi-square, P=0.0312 (grip2a: n=2 females for each genotype; wnt8a: n=4 Mkif5Ba and n=3 WT females). (O-′′) Sybu is vegetally localized at 2mpa (O) and is asymmetric at 45mpa (P) in WT activated eggs. This is normal in some Mkif5Ba mutant eggs (Q,R), but others show aberrant localization at 2mpa (Q′,Q′′) and at 45mpa (R′,R′′). See text for quantification. (S) Injection of ha-kif5Ba RNA into 1-cell-stage Mkif5Ba mutants fails to suppress DV patterning defects. [n=138 uninjected and n=81 injected Mkif5Ba^ae11/ae12 (N=1 female), n=172 uninjected and n=80 injected Mkif5Ba^ae11/ae12 (N=2 females)]. Arrowheads designate the lateral limits of each expression domain.