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Fig. 2
Dotty expression of γ-tubulin at the non-mitotic nuclei does not derive from enhanced background. (A) Higher magnification of the framed area shown in Fig.1 C labeled with PH3, γ-tubulin and DAPI. (B) The simultaneous increase of the green and red input levels reveals the dotty pattern of only γ-tubulin (green) but not PH3 (red). This demonstrates the specificity of green immunofluorescence consistent with the centrosomal localization of γ-tubulin. Scale bar (A)=25 µm; S=shadows; M=midtones; H=highlights.
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