Fig. 3
NTR-mediated RGC ablation in Tg(gfap:Gal4FF;UAS:nfsB-mcherry) adult zebrafish. a: Mtz treatment schedule for RGC-specific ablation. b–d: Expression of mcherry and GFAP in the telencephalon of adult Tg zebrafish after Mtz treatment. Sections of the telencephalon of Tg fish were stained with anti-GFAP (green) antibody. Nuclei were stained with Hoechst (blue). e–g: Expression of mcherry and GFAP in the telencephalon of adult Tg(gfap:Gal4FF;UAS:nfsB-mcherry) zebrafish. Sections of the telencephalon of Tg fish were stained with anti-GFAP (green) antibody. Nuclei were stained with Hoechst (blue). i–l: The number of mcherry-positive cells in the adult telencephalon with or without the Mtz treatment. Dm, Dl, and Vd. *P < 0.05. Scale bars, 200 µm in b and e, 100 µm in b′ and e′.