ZFIN Image: Xue et al., 2015, Fig. S5

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Figure Caption

Fig. S5 Validation of klf6a MO efficiency and expression of klf6a predicted target genes.

(A) Western blot was used to test the Klf6 protein level in klf6a morphants. The results showed that Klf6 was decreased remarkably after knocking down klf6a at 2 dpf. (B) The expression of tf and tfR was reduced in klf6a morphants (mean ± SD, t-test, ***P<0.001, **P<0.01, n = 3). (C, D) The qPCR result and O-dianisidine staining displayed that the be1-globin expression and hemoglobin staining were rescued when co-injected klf6a mRNA compared to klf6a morphants (mean ± SD, t-test, ***P<0.001, *P<0.05, n = 3). (E) The WISH profiles included bcl2-l10, mcl1b and NF-KBiA-b showed no difference in controls and morphants at stages 24-25 hpf.

Acknowledgments

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Reprinted from Developmental Biology, 403(2), Xue, Y., Gao, S., Liu, F., Genome-wide Analysis of the Zebrafish Klf Family Identifies Two Genes important for Erythroid Maturation, 115-27, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.