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Fig. S4
Hand2:GFP, myl7:hand2-mCherry and fli1a:hand2-mCherry reporter expression.
(A,B) Fluorescent imaging of cardiac region in hand2:GFP; kdrl:mCherry transgenic embryos at 35 hpf. (A) kdrl:mCherry+ endocardial cells are shown in red and hand2:GFP+ myocardial cells in green. (B) GFP channel shows both myocardial (white arrowhead) and endocardial cells (red arrowhead) labeled with hand2:GFP. (C) Mosaic expression of injected myl7:hand2-mCherry construct overlaps with Tg(myl7:GFP) fluorescence at 36 hpf. Fluorescence in lightly fixed embryos is shown. (D) mCherry fluorescence is observed in the vasculature and the endocardium at 30 hpf in lightly fixed Tg(fli1a:hand2-mCherry); Tg(myl7:GFP) embryos.
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