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Fig. S3
fn1:mCherry-NTR expression in han mutants and their wild-type siblings.
Confocal microscopy images of live myl7:GFP; fn1:mCherry-NTR (A-F) and etv2:GFP; fn1:mCherry-NTR (G-L) transgenic embryos at the 22-24-somite stages. Maximum intensity projections of GFP (A,D,G,J), mCherry (B,E,H,K) and merged channels (C,F,I,L) are shown. Note that the myocardial myl7:GFP expression has only limited overlap with the endocardial fn1:mCherry expression at the midline (arrow, B,C) in wild-type (wt) embryos, while endocardial fn1:mCherry expression is reduced in han mutants (arrow, E,F), which exhibit bilateral domains of intense fn1:mCherry expression (arrowheads, E,F). Endocardial etv2:GFP expression largely overlaps with the midline domain of fn1:mCherry expression (arrows G-I) in wt embryos, while han mutants exhibit bilateral fn1:mCherry expression domains (arrowheads, K,L) which significantly overlap with etv2:GFP expression. Dorsal views, anterior is to the top.
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