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Fig. S2
Fibronectin expression dynamically labels both endocardial and myocardial cells.
(A) Analysis of fn1:mCherry-NTR; myl7:GFP expression at the 17-somite stage. Note that there is a partial overlap between the bilaterally located fn1:mCherry-NTR and myocardial myl7:GFP expression while mCherry-positive cells present at the midline correspond to the presumptive endocardial progenitors and are GFP-negative.
(B,C) kdrl:GFP; fn1:mCherry-NTR expression in the cardiac region at the 21-somite (B) and 22-somite (C) stages. Note that in (B) the cardiac plate is arranged with kdrl:GFP labeled endocardial cells in the middle (B, 3rd & 5th column, green outline) and fn1:mCherry-NTR labeled myocardial cells (B, 4th & 5th column, red outline) on the outer periphery of the cardiac plate. Endocardial cells co-expressing both kdrl:GFP and fn1:mCherrry-NTR (B, 5th column, yellow outline) are generally located at the interface/border between endocardium and myocardium. At the 22-somite stage, the cardiac plate rearranges to begin cardiac cone extension (C). During this complex cellular rearrangement, yellow endocardial cells co-expressing both kdrl:GFP and fn1:mCherry-NTR (C, 5th column) are distributed throughout the growing cone.
(D,E) kdrl:GFP; fn1:mCherry-NTR expression in the cardiac region at 42 hpf. Higher magnification view of the ventricle and outflow tract is shown in (E). Note that fn1:mCherry-NTR expression is more robust in the myocardial layer than in the endocardial layer, especially at the ventricular pole. However, there is a subtle but present expression of fn1:mCherry-NTR (3rd column) in the inner endocardial layer (4th column) of the heart (yellow arrowheads).
(A-C) are whole embryo, ventral flat mount views. (D,E) are whole embryo, anterolateral views. All panels show GFP and mCherry fluorescence in fixed embryos.
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