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Fig. S1
Loss of Hand2 function prevents endocardial cone morphogenesis and causes aberrant midline migration of etv2:GFP cells.
(A-C) Double transgenic hearts labeled red for myocardium (myl7:mCherry, left columns) and green for endothelial/endocardial cells (etv2:GFP, middle columns) in wild-type (wt, A-C) and han-/- (D-F) embryos at 22 hpf (A and D), 26-29 hpf (B and E), and 30-36 hpf (C and F). All panels are fluorescent images of GFP and mCherry fluorescence in fixed and flatmounted embryos, ventral views. Dotted outlines indicate endocardial cones (A-C) or presumptive endocardial precursors (D-F). Note the inflow tract connecting to the first aortic arch in wt embryos (arrowheads, A-C) and ectopic vessel extending posteriorly from the endocardial sheet in han-/- mutants (arrowheads, D-F).
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