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Fig. 3
Notch-1a mRNA expression. (a) Notch-1a expression is dramatically increased in 2-dpf Ang-1 MO morphant brains and 6-dpf brains in the pallium, thalamus, medial and later domains of tectum opticum and intermediate and caudal hypothalamus (n = 6–8 each group). The Ang-1 mRNA normalizes the overexpression of Notch-1a in Ang-1 MO morphants. (b) qPCR analysis of Notch-1a transcript levels (*p < 0.05, n = 9, one-way ANOVA with Dunnett′s test). DT, dorsal thalamus; Hc, caudal hypothalamus; Hi, intermediate hypothalamus; OB, olfactory bulb; P, pallium; RL, rhombic lip; TeO, tectum opticum; VT, ventral thalamus. Scale bar, 100 µm.