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Fig. 5
INF2 knockout in zebrafish results in slit diaphragm dysfunction. Control and INF2 morphants were examined for the effect of INF2 knockdown on the gross phenotype (a) and histology of glomerulus (G) (b). At 96 hpf, FITC-labeled dextran was injected into the cardinal vein of the fish. 24 h later, fish were sacrificed and sections at the kidney plane were examined under light microscopy (b) and fluorescence microscopy (c). Uptake of injected FITC-dextran in tubular cells indicates the increased leakage of molecules through the glomerular filtration barrier with INF knockdown (d. magnified tubular cells, INF2 MO vs. Control MO). G denotes glomerulus, T denotes tubule.