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Fig. 4
INF2 knockout leads to altered podocyte structure and nephrin mistrafficking. a. Ultrastructure of glomerulus and podocyte in INF2 morphant zebrafish vs. control fish. b. Cultured podocytes were transfected with INF2-targeting siRNA or a control RNA duplex (control siRNA) for 48 h. Then the cells in both groups were transfected with a plasmid encoding human nephrin. 24 h later, nephrin expression was illustrated by immunofluorescence stain in cultured podocyte with INF2 knockdown (INF2 siRNA) vs. control cells (control siRNA). c. Glomerular section of INF2 morphants (INF2 MO) and control fish (control MO). The expression of nephrin is illustrated using immunofluorescence staining in the zebrafish glomerulus (INF2 morphants (INF2 MO) vs. control fish (control MO)).