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Fig. 1, S2 Abrogation of Shh signaling abolishes vegfaa expression, but does not influence angioblast migration.
(A, B) Embryos treated with 100 µM Cyclopamine (CyA) from 11 hpf to 18 hpf to block Sonic hedgehog signaling showed no defects in medial angioblast migration. Confocal projections of Tg(fli1a:EGFP)y1 embryos in dorsal views at 18 hpf. c-d) vegfaa expression in the somites is abolished in embryos treated with 100 µM Cyclopamine (CyA) from 11 hpf to 18 hpf.
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