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Fig. S4 Overexpression of id1-mRNA mimics the phenotype in mecp2 morphants while id1-MO mimics that in embryos over-expressing mecp2 mRNA. RNA in situ hybridization with nestin, gfap, map2, and neurod probes in controls (A-D), embryos injected with id1-mRNA (A′-D′), embryos injected with mecp2-mRNA (A′′-D′′), and id1 morphants (A′′′-D′′′) at 48 hpf. The expression of these genes in the developing brain of embryos injected with id1-mRNA was similar to those in mecp2 morphants. The expression of nestin and gfap were down-regulated, while map2 and neurod were up-regulated in either embryos injected with mecp2-mRNA or id1 morphants. Dorsal views with anterior to the left. The numbers in the bottom right corner represent phenotypic embryos/total embryos. (E-G) Quantitative real-time PCR analysis of nestin, gfap, map2, and neurod gene expression in wild-type embryos along with embryos injected with mecp2-mRNA (E), id1 morphants (F), and embryos injected with id1-mRNA (G), normalized to gapdh. Measurements are the means from 3 independent experiments ± SD. *P <0.05.