Fig. S2
Supplemental figure 2. Chemically induced inflammation (ChIn) assay.
DMSO-treated Tg(mpx:EGFP) larvae show the normal distribution of labeled cells, mostly localized in the ventral trunk and tail (A). In copper-treated siblings (B), leukocytes become localized preferentially to a few clusters along the horizontal midline of the trunk and tail (white arrows). Larvae exposed to indicated compounds (C-H) exhibit a decreasing number of leukocytes along the PLL. Inhibitors (C-H) were added to the incubation medium 30 min prior to addition of copper and were tested for inhibition of leukocyte migration using ChIn assays.