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Fig. S3
Reporter mRNA injections do not affect development. Venus (variant of YFP) mRNA was injected into the yolk of embryos at the one-cell stage. The injection concentration used was 167 ng/uL. About 2 nL was injected into one group, which is equivalent to about ~330 ng (n=47). About 4 nL (double the amount) was injected into a second group, which is equivalent to ~660 ng (n=34). All embryos injected fluoresce GFP. Representative images were taken at 24 hpf.
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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.