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Fig. S2
Un-injected heat-shocked embryos do not display growth defects. Un-injected embryos (n>20 for each group) were heat-shocked at either 24 hpf or 48 hpf for 60 min at 37 °C followed by 60 min at 28.5 °C and this was repeated for a total of three times. Embryos were then allowed to develop at 28.5 °C until the stage of analysis. Controls were not heat-shocked, but were derived from the same clutches and raised at 28.5 °C.
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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.