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Fig. 9 Photo-Morpholinos cause rhodopsin mislocalization and loss of rod photoreceptors. (A, E) Transverse retinal cryosections from 4 dpf wild-type; (B, F) pard3MO-pard3pMO; (C, G) prkciMO-prkcipMO; (D, H) prkci/zMO-prkci/zpMO injected larvae. Embryos were injected with MO/S-photo-MO hybrids and irradiated at 30 hpf with UV light. Immunohistochemistry was performed on transverse cryosections of 4 dpf larvae and stained with 1D1 to label rhodopsin (green) and phalloidin (red) and counterstained with DAPI to label nuclei. Bottom row shows 1D1 and DAPI staining only. (I) Quantification of total 1D1+ rod photoreceptors in 10 micron cryosections. (*** = p≤0.0001) ONL = outer nuclear layer; INL = inner nuclear layer. Scale bar = 100 μm (A–D) or 20 μm (E–H).