ZFIN Image: Bührdel et al., 2015, Fig. 3

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Fig. 3

Disturbed myofibrillar structure in MFM gene deficient embryos (A–C) Lateral views of a control zebrafish (A) at 72 hpf showing bright homogenous birefringence compared to reduced birefringence in Desmin-a (B) and DNAJB6-b (C) morphants. (D) Quantification of birefringence in Desmin-a and DNAJB6-b morphants. (E–L) Electron microscopy of MFM gene deficient and control embryos at 72 hpf. (E) Nuclei (dotted arrows) in the skeletal muscle are elongated in controls; (F,H) rounded nuclei in myopathic muscle. (I) Control injected embryos develop highly ordered myofibrils with ordered sarcomeric units, Z-disks, M-Bands and A/I regions. (F,H) Vacuolization in MO-Desma and MO-crayaba injected embryos (asterisk). (G) Autophagic pathology in Dnajb6-a morphants. (F,J,K) Mitochondrial alteration in Desmin-a, Plectin-b and αB-crystallin-a morphants (arrowheads). (L) Stress fiber-like structures in Myotilin morphants (arrows). Scale bars 1 µm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.