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Fig. 5
Mesonephric expression of early and mature nephron markers. A,B: Whole mount in situ hybridizations of larvae show that the early acting transcription factor wt1b is expressed in clusters of nephron progenitor cells (arrows and arrowheads). C: The mature glomerular marker nphs1 is expressed in mesonephric glomeruli (white arrows and arrowheads) in addition to being maintained in the PG (black arrow). D–F: Magnified images of nephron progenitor clusters expressing the early acting markers wt1b, pax2a, and lhx1a. G–H: Magnified images showing a mature glomerulus (G) and an immature presumptive glomerulus (H) expressing nphs1 (arrows). PG: pronephric glomerulus.