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Fig. 4
Mesonephric expression of the pan-tubule cdh17 marker. A–G: Whole mount in situ hybridizations of larvae show that the first mesonephric nephron expresses cdh17 at the 5.2–5.5 mm stage (B white arrow and inset). Additional nephrons appear later in both caudal and rostral positions relative to the first nephron (C,D: white arrows, arrowheads, and inset). The inset in C is a magnified image of the arrowhead. At the 9 mm stage (<30 dpf), the developing mesonephros has three distinct regions (head, trunk, and tail; F,G), resembling the adult mesonephros in H. H: The mesonephros of a sexually mature adult fish (90 dpf) has the head, trunk, and tail regions. SB: swim bladder; G: glomerulus.