ZFIN Image: Huang et al., 2015, Fig. 6

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Figure Caption

Fig. 6

Disrupted Kmt2a expression causes decreased glial precursors and derivatives. The expression of slc1a3a, oilg1, and mag were analyzed by in situ hybridization (A), and Gfap was examined in Tg(gfap:egfp) transgenic embryos (C). Injection of the kmt2a morpholino or kmt2a^N84 cRNA downregulated the expression of all these glial markers. (B) qPCR analysis confirmed the results obtained by in situ hybridization in A. (D) Levels of GFP expression in C were confirmed by Western blot analysis and were quantified. Quantitative data are presented as the means ± standard deviations. *p < 0.05; **p < 0.01; n.s., not significant.

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Acknowledgments

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