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Figure Caption
Fig. S4
Effect of l-NAME on morphogenetic movements of interrenal steroidogenic tissue and IRV formation.
(A–A′′) Tg(kdrl: GFP)s843 embryos treated with 100 µM l-NAME from 1.5 dpf onwards had interrenal steroidogenic tissue (IR) morphology (orange arrows) and (B–B′′) IRV length similar to control embryos (Figure 3A, A′, D) at 2.5 dpf (n = 8). The activity of endothelial NO synthase was inhibited by l-NAME at concentrations higher than 10 µM [50]. D, dorsal; V, ventral; L, left; R, right. Abbreviations: posterior cardinal vein (PCV). Scale bar, 50 µm.
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