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Fig. 4, S3
wnt10a, fgf3 and fgf10 expression are induced within interneuromast cells after Wnt/β-catenin activation.
Wild type zebrafish were treated with DMSO or BIO at 48 hpf then fixed at 6, 12 or 24 hr post treatment for in situ hybridizations. The known Wnt/β-catenin target gene lef1 is expressed in DMSO treated interneuromast cells (A, C, E) and is greatly increased by BIO treatment (B, D, F). wnt10a is not expressed in control interneuromast cells from 6 to 24 hr post treatment (G, I, K). wnt10a is induced by BIO within 6 hr post (H) and this increase is maintained from 12 to 24 hr post (J, L). fgf3 (M, O, Q) and fgf10 (S, U, W) are not expressed within DMSO treated interneuromast cells. fgf3 is induced by 24 hr post BIO treatment (N, P, R), while fgf10 is induced within 12 hr of treatment (T, V, X).