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Fig. S4
miR-216a does not target her4.2, notch1b, heyl, numb, or hey2. Embryos were injected at the 1-cell stage with GPF reporters containing the 3'UTRs of her4.2, notch1b, heyl, numb, or hey2 alone or in combination with miR-216a. Embryos grown in egg water until 24 hpf, at which point they were photographed using a fluorescence dissecting scope. We did not detect changes in GFP fluorescence with co-injection of miR-216a.
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Reprinted from Developmental Biology, 400(1), Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G., miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development, 72-81, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.