IMAGE

Fig. 2

ID
ZDB-IMAGE-150422-48
Source
Figures for Olena et al., 2015
Image
Figure Caption

Fig. 2

snx5 is a target of miR-216a. The coding sequence of GFP was fused to the 32UTR of snx5. Predicted pairing of each MRE in the 32 UTR (black) and miR-216a (red) are pictured. (B) Embryos injected at the 1-cell stage with GFP-snx5 32 UTR reporter mRNA alone, with miR-216a (C), or with miR-216a and miR-216aMO (D) were imaged using a fluorescence dissecting scope at 1 dpf. (F) Both MREs were deleted from the GFP-snx5 32 UTR reporter. Embryos injected at the 1-cell stage with this mRNA alone, with miR-216a (G), or with miR-216a and miR-216aMO (H) were imaged at 1 dpf using a fluorescence dissecting scope. (E, I) Relative fluorescence was quantified using ImageJ, and comparisons were made using one-way ANOVA with BonferroniĆ³s correction. (J) Western blots for SNX5 and alpha-tubulin were performed on protein lysates from 1 dpf zebrafish injected at 1-cell stage with dye control (DIC), miR-216a, or miR-216aMOs. (K) Band density was quantified using ImageJ, and comparisons were made using one-way ANOVA with BonferroniĆ³s correction. *, p<0.05; **, p<0.01; ***, p<0.001. Error bars show SEM.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 400(1), Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G., miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development, 72-81, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.