ZFIN Image: Kok et al., 2015, Fig. 1

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Figure Caption

Fig. 1

TALEN-Generated Mutations in ccbe1 and flt4 Cause Lymphatic Defects

(A) Schematic of Ccbe1 protein indicating position of the ccbe1^hu10965 frameshift. SP, signal peptide; EGF, EGF-like domain; Ca²⁺-EGF, calcium-binding EGF-like domain; and collagen, collagen-like domain. Images are confocal micrographs of wild-type or ccbe1^hu10965 mutant embryo bearing Tg(flt4:mCitrine)^hu7135 (green) and Tg(flt1:tdTomato)^hu5333 (red) transgenes. Homozygous ccbe1^hu10965 mutants lacked the TD and ISLVs at 5 days postfertilization; position of the DA and posterior cardinal vein (PCV) are indicated. Lateral views, anterior to the left, and dorsal is up.

(B) Confocal micrographs of Tg(fli1a:egfp)^y1 wild-type and flt4^um203 sibling embryos at 24 hrpf, 30 hrpf, 2 days postfertilization, and 5 days postfertilization. Positions of the PHBC, ISVs, PLs, and TD are indicated. All of these structures except ISVs are missing in flt4^um203 mutants at indicated stages (right). (Bottom) flt4 transcript by whole mount in situ hybridization in wild-type and flt4^um203 mutant embryos at 28 hrpf. Lateral views, anterior to the left, and dorsal is up.

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Acknowledgments

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Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell