ZFIN Image: Phng et al., 2015, Fig. 4

Image

Figure Caption

Fig. 4

Formin Activity Is Essential for Junction Stability

(A and B) Still images of VEC-EGFP-expressing HDMEC treated with 0.02% DMSO or 20 µM SMIFH2. Imaging began 5 min after addition of SMIFH2. A′ and B′ show linear junctions. A′′ and B′′ show FAJs. The arrowheads show gaps within linear junctions, and arrows show the direction of FAJ displacement. Scale bars represents 20 µm.

(C–F) VEC and ZO1 immunostainings in ISVs and DLAV of control and fmnl3 morphants at 53 hpf (C and D) and in 2 dpf Tg(fli1a:EGFP)^y1 embryos after 2 hr treatment with DMSO or 10µM SMIFH2 (E and F). Boxed regions in C and D show only endothelial staining. The arrows show junctional gaps. Scale bars represent 20 µm.

(G) Quantification of junctional gaps within ISVs at 0, 1, 2 or 3 hr after 10µM SMIFH2 treatment. Data represent mean ± SD.

See also Figure S4 and Movie S4.

Acknowledgments

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Reprinted from Developmental Cell, 32, Phng, L.K., Gebala, V., Bentley, K., Philippides, A., Wacker, A., Mathivet, T., Sauteur, L., Stanchi, F., Belting, H.G., Affolter, M., Gerhardt, H., Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization, 123-32, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell