ZFIN Image: Ceci et al., 2014, Fig. S5

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Figure Caption

Fig. S5 Loss of Schwann cell differentiation markers after denervation at 5 dpf. Five-day-old tg(foxD3:GFP)/tg(NeuroD:RFP) double transgenic larvae were left untreated or were neurectomized and observed 2 dpn. In these fish, Schwann cells are labeled by green and the nerve by red fluorescence. In neurectomized fish, at 48 hpn, the regrowing nerve almost reaches the tip of the tail (arrowhead in (A), inset). At the same time, a distal decrease in GFP expression is observed (B) compared to age-matched non-neurectomized controls (C) (compare insets that show enlarged image of trunk and tail). The same experiment carried out with 3-day-old fish showed a similar result. The dotted square in (D) shows the area of the fish imaged in (E, F). E shows Schwann cells in a larva 24 hpn; F shows the same area in a control larva. Scale: E, F: 200 µm; A-C, inset in B, inset in C: 100 µm.

Acknowledgments

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