ZFIN Image: Lu et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

Mouse Arl13b mRNA can rescue the zebrafish arl13b morphant phenotype. (A) Mouse Arl13b mRNA rescued the shortening of cilia in arl13b morphants. Cilia on somitic cells are shown. (B) Quantitation of somitic primary cilia length in wild-type, arl13b morphants and arl13b morphants with mouse Arl13b overexpression. (C) Curled down body of arl13b morphant zebrafish is rescued by mouse Arl13b mRNA. Ciliary axonemes were labeled with anti-acetylated tubulin antibodies (red) and mouse Arl13b was visualized with GFP fluorescence (green). In this and other figures, nuclei were labeled with DAPI (blue), scale bars=1 µm and error bars indicate SEM, unless mentioned otherwise.

Acknowledgments

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Reprinted from Developmental Biology, 397(2), Lu, H., Toh, M.T., Narasimhan, V., Thamilselvam, S.K., Choksi, S.P., Roy, S., A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation, 225-36, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.