Fig. S2

Fig. S2 Smad3-regulated EGFP expression is independent from Smad1/5/8-BMP and partially dependent on Nodal signaling. A–D′′′, brightfield and fluorescent lateral views of embryos at 24 hpf. Anterior to the left. A–A′′′: wild type (WT) 24 hpf embryo of the Tg(12xSBE:EGFP)ia16 line. B–B′′′, 24 hpf one-eyed pinhead (oep) mutant of the Tg(12xSBE:EGFP)ia16 line. C–C′′′: 24 hpf chordin (chd) mutant embryo of the Tg(12xSBE:EGFP)ia16 line. A′′′, B′′′, C′′′, arrow head indicates the cardiac mesoderm region. GFP expression is absent in oep mutants. D–D′′, E–E′′, brightfield and fluorescent images of lateral view of 72 hpf larvae of 12xSBE line treated at 24 hpf with DMSO (D–D′′) or LY364947 (E–E′′), anterior to the left. D′′′, E′′′, in situ hybridization lateral view images of a 48 hpf larva treated with DMSO (D′′′) or LY364947 (E′′′) at 24 hpf.