Fig. 3

Fig. 3 Tg(12xSBE:nls-mCherry)ia15 line is responsive to smad7 and smad3b overexpression. A–C′′, confocal lateral views (Z-stacks) of 48 hpf double transgenic larvae, Tg(12xSBE:nls-mCherry)ia15/(hs-Smad7/YFP) or (hs-Smad7/YFP). As shown in the schematic 48 hpf larva, three areas have been analyzed: head, trunk and tail. Each picture shows the merge of YFP (green) and mCherry (red) and it is accompanied by a small figure (white square) representing mCherry fluorescence following the heat-shock. (A–A′′) Confocal lateral views of 48 hpf double transgenic larvae, Tg(12xSBE:nls-mCherry)ia15/(hs-Smad7/YFP). Heat-shock causes a downregulation of the 12xSBE reporter (in red) and ubiquitous production of YFP (in green). (B–B3) Confocal lateral images of 48 hpf non-heat-shocked double transgenic larvae, Tg(12xSBE:nls-mCherry)ia15/(hs-smad7/YFP) and Tg(12xSBE:nls-mCherry)ia15/(hs-smad3b/YFP). Only non-heat-shocked Tg(12xSBE:nls-mCherry)ia15/(hs-smad7/YFP) is shown as a control. (C–C′′) Confocal lateral views of 48 hpf heat-shocked double transgenic larvae, Tg(12xSBE:nls-mCherry)ia15/(hs-smad3b/YFP). The heat-shock leads to a smad3b overexpression confirmed by mosaic production of YFP. White arrow heads show ectopic expression in the head, trunk muscle and tail. (D–D′′) Zoomed views (single planes) of Tg(12xSBE:nls-mCherry)ia15/(hs-smad3b/YFP) highlight the ectopic reporter expression induced by smad3b overexpression. Scale bar is 100 µm in A–C′′ 20 µm in D–D′′. Scale bar is 100 µm in A–B′, E–F′, G, H; 50 µm in C′–C′′ and 20 µm in G′, H′.