Fig. S5

Fig. S5 Tg(12xSBE:EGFP)ia16 line is responsive to the overexpression of smad3b and in addition, smad3a together with smad3b: effects of mRNA injection on embryos with a normal phenotype. Brightfield and fluorescent images of head and tail of 24 hpf embryos injected with smad2, 3a, 3b and 4 mRNAs. The last column shows zoomed view of the tails. White arrow heads in fluorescent head pictures point out the cardiac mesoderm. Injection of smad3b and smad3a+smad3b mRNAs causes an increase of GFP expression in this area. In the same injected embryos overexpression of GFP is also visible in tail mesoderm (white arrow heads). Overexpression of both smad3 isoforms results in an ectopic GFP production in notochord (zoomed view of GFP+ notochord is surrounded by a white square).