ZFIN Image: Angers et al., 2014, Fig. 2

Image

Figure Caption

Fig. 2 Expression of itch genes in zebrafish embryos.

(A) RT-PCR experiment showing early expression of both itcha and itchb genes. RNA was extracted from embryos at approximately 6, 24 and 48 hpf. cDNA was obtained from each stage using 1 µg total RNA. GAPDH was used as an internal control for cDNA amplification. (B–D) Weak, general staining of embryos at 26 hpf revealed with DIG-labelled probes complementary to itcha (B) or itchb (C) or sense sequence (D) as a control. (E–G) The primordium of 26 hpf embryos revealed with DIG-labelled probes complementary to itcha (E) or itchb (F) or sense sequence (G) as a control. Position of the primordium is underlined with a dotted line. Expression of itch genes is general, and itchb is expressed throughout the primordium. Scale bar: (B–D) 250 µm, (E–G) 25 µm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One