Fig. 9

Fig. 9 Muscle and bone in jef mutants. Expression of titin in wild-type (A) and homozygous jef^tw37 (B) and jef^hi1134 (C) animals at 3 dpf reveals normal patterning of cranial muscles. Compiled stacks of confocal micrographs of wildtype (D) and a jef^tw37 mutant (E) stained with calcein. Most dermal bones (op, bsr, cl) are relatively unaffected in jef mutants, whereas the dermal dentary (de) and cartilage replacement fifth ceratobranchial bone (cb5) are severely reduced. Teeth (te) are present, as are tiny remnants of bone at the base of the teeth. Bone development occurs on schedule without enlarged ossification centers in jef mutants. am, adductor mandibulae; bsr, branchiostegal ray; cb5, fifth ceratobranchial; cl, cleithrum; de, dentary; hh, hyohyoideus; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; op, opercle; sh, sternohyoideus; te, teeth; tv1, transversus ventralis. Scale bar: in A, 100 μm for A-C.