ZFIN Image: Schindler et al., 2014, Fig. S2

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Fig. S2

Induction of hand2 overexpression at 10 hpf, but not at 24 hpf, produces a morphologically evident cardiac phenotype. (A-F) Lateral views of live embryos at 36 hpf depict (A-C) embryonic morphology and (D-F) mCherry fluorescence in (A,D) a nontransgenic embryo and (B,C,E,F) embryos carrying the transgene Tg(hsp70:FLAG-hand2-2A-mCherry) (abbreviated as Tg(hsp70:hand2)), after heat shock at (A,B,D,E) 10 hpf (tail bud) or (C,F) 24 hpf. Heat shock of transgenic embryos at 10 hpf leads to pericardial edema (B), and residual mCherry fluorescence is still evident in these embryos at 36 hpf (E). In contrast, edema is not observed in transgenic embryos after heat shock at 24 hpf (C); mCherry fluorescence remains pervasive in these embryos at 36 hpf (F).

Acknowledgments

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