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Fig. 12 Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GGATCAATCAGCAGCAGAGAAAC. Reverse: AATTAACCCTCACTAAAGGGAGGTCATCCTTTACATGCAGAGC. PCR product size: 1055 bp.
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