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Fig. 6 Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ATCGAGAGCACACCCGACAAAAG. Reverse: AATTAACCCTCACTAAAGGGACACTAAGTGTTGAAGTGGAAGAGC. PCR product size: 457 bp. Embryos have been over-developed to detect all possible expression.
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