ZFIN Image: Kanagaraj et al., 2014, Fig. 5

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Figure Caption

Fig. 5

Suf/Spastizin and Rab11b colocalize on cortical granules.

Optical sections of stage III oocytes showing the localization of (A, A2) Rab11b (green) or (B, B2) Suf/Spastizin (green) on cortical granules (CG) labeled with MPA-lectin (red). Scale bar: 50 μm. Stippled boxes in A, B highlight magnified area in A2, B2. (C–E) Rab11b and Suf/Spastizin colocalize in central, optical sections on cortical granules. Confocal section showing colocalization of Rab11b (green) (C) or Suf/Spastizin (green) (D) on cortical granules (red) in wild-type oocytes. (E) Upper row: Single channels of Rab11b (green; left panel) and MPA-lectin (red; center panel) showing localization of Rab11b on cortical granules (yellow; right panel). Lower row: Single channels of Suf/Spastizin (green) on cortical granules (red) forming a polarized microdomain on the granule membrane (yellow). Scale bar: 20 μm.

Acknowledgments

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