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Fig. S3
Tg(fabp10:nAtf6-cherry; cmlc2:GFP) larvae express the nAtf6-cherry transgene despite a lack of visual fluorescence. A: Schematic of human ATF6 transactivation (TAD), DNA-binding (bZIP) and transmembrane (TM) domains, cleavage sites for S1P (MBTPS1) and S2P (MBTPS2), and protein alignment with zebrafish Atf6. Numbers in the diagram correspond to amino acid positions in human ATF6. Numbers at the cleavage sites in the protein alignment correspond to amino acid positions in zebrafish Atf6. B: Confirmation of nuclear localization of zebrafish nAtf6 by transfection of nAtf6-GFP/pCI-Neo into HepG2 cells. C: Live image of Tg(fabp10:nAtf6-cherry; cmlc2:GFP) larva at 5 dpf. D: Agarose gel electrophoresis showing expression of Cherry mRNA in nAtf6 TG but not WT larvae. rpp0 was used to ensure PCR efficacy. E: Western blot visualization of Cherry protein in adult nAtf6 transgenic zebrafish liver tissue. Tg(fabp10:nls-cherry) adult liver protein was used as a positive control. Actin was used as a loading control.