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Fig. 5
The PI3K/mTOR pathway contributes to the blockage of myeloid cell maturation in pten-/- embryos. (A-F) Sudan Black staining of neutrophils at 90 hpf. Control and pten-/- embryos were treated with DMSO (A-B) or LY294002/Rapamycin (C-D and E-F) at indicated concentration. The red arrowheads indicate neutrophils in the CHT. (G) statistical results of A-F. The data shown in (G) are the means ± SEM of at least 30 embryos; ***p < 0.001 versus the control. Scale bar: 100 μm.
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Full text @ J. Hematol. Oncol.