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Fig. S6
ColI deposition at the amputation site in def+/- is not mediated by the reactive oxygen species signalling pathway. Representative images of co-immunostaining of ColI (red) and Bhmt (green) in the wound epidermis in wild-type, def+/- and def+/- fish treated with diphenyleneiodonium (DPI) or apocynin (APO), two inhibitors of the reactive oxygen species signalling, 5 days after PH. Nuclei were stained with DAPI (blue). lv, liver tissue; we, wound epidermis. Scale bar: 75 μm.
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