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Fig. 4
Def haploinsufficiency results in accumulation of ColI at the amputation site after PH.
(A,B) Representative images of immunostaining of keratin 18 (green) (A) and co-immunostaining of ColI (red) and Bhmt (green) (B) at the amputation site in wild-type and def+/- strains 3 days after PH. Nuclei were stained with DAPI (blue). At each time-point, more than 10 sections from at least three wild-type or def+/- mutant fish were examined. lv, liver tissue; we, wound epidermis. Scale bars: 75 μm (A,B) and 100 μm (C).
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